Construction of a luks-pv mutant of a staphylococcal panton-valentine leukocidin component having a high luks-like function

Akiko Shimatani, Jun Kaneko, Toshio Tomita, Yoshiyuki Kamio

Research output: Contribution to journalArticlepeer-review

Abstract

A 2-residue (D12I13) segment of LukS of a staphylococcal leukocidin component is an essential region for the hemolytic function of LukS towards rabbit erythrocytes in the presence of LukF. Here, we report that insertion of D, I, or AA residue(s) between A11 and E12 residues of LukS-PV, in which the 2-residue D12I13 segment in LukS was absent, confers the full LukS function on LukS-PV, which has only 4% hemolytic activity of that of LukS towards rabbit erythrocytes.

Original languageEnglish
Pages (from-to)1828-1830
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number10
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • LukF-PV
  • LukS-PV
  • Panton-Valentine leukocidin
  • Staphylococcal leukocidin

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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