Construction and analysis of full-length cDNA library of Cryptosporidium parvum

Junya Yamagishi, Hiroyuki Wakaguri, Sumio Sugano, Suguru Kawano, Kozo Fujisaki, Chihiro Sugimoto, Junichi Watanabe, Yutaka Suzuki, Isao Kimata, Xuenan Xuan

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    A full-length cDNA library was constructed from the sporozoite of Cryptosporidium parvum. Normalized clones were subjected to Solexa shotgun sequencing, and then complete sequences for 1066 clones were reconfigured. Detailed analyses of the sequences revealed that 13.5% of the transcripts were spliced; the average and median 5′ UTR lengths were 213.5 and 122 nucleotides, respectively. There were 148 inconsistencies out of 562 examined genes between the experimentally described cDNA sequence and the predicted sequence from its genome. In addition, we identified 118 sequences that had little homology against annotated genes of C. parvum as prospective candidates for addable genes. These observations should improve the reliability of C. parvum transcriptome and provide a versatile resource for further studies.

    Original languageEnglish
    Pages (from-to)199-202
    Number of pages4
    JournalParasitology International
    Volume60
    Issue number2
    DOIs
    Publication statusPublished - 2011 Jun 1

    Keywords

    • Cryptosporidium
    • Full-length cDNA library
    • Next generation sequencing
    • Transcriptome

    ASJC Scopus subject areas

    • Parasitology
    • Infectious Diseases

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  • Cite this

    Yamagishi, J., Wakaguri, H., Sugano, S., Kawano, S., Fujisaki, K., Sugimoto, C., Watanabe, J., Suzuki, Y., Kimata, I., & Xuan, X. (2011). Construction and analysis of full-length cDNA library of Cryptosporidium parvum. Parasitology International, 60(2), 199-202. https://doi.org/10.1016/j.parint.2011.03.001