Constructing a multi-scan synchrotron X-ray microscope to study the function of osteocyte canaliculi in mouse bone

Nobuhito Nango, Shogo Kubota, Wataru Yashiro, Atsushi Momose, Yasunari Takada, Koichi Matsuo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Formulating a multi-scan method applied to an X-ray microscope CT with synchrotron radiation, we attempted to analyze the 3D functional structure of osteocyte canaliculi inside the cortical bone of a mouse tibia. We employed a two-method combination to scan the same position of the specimen. To extract the internal bone canalicular structure, we first combined a Talbot interferometer with an X-ray microscope, and applied a differential phase imaging method to measure the absolute value of bone mineral around the canaliculi. Next, we used the X-ray microscope without the Talbot interferometer under a defocus condition, moving the specimen toward the zone plate by 6 mm. This defocus contrast method visualizes the canaliculi by emphasizing the edges of the bone. We performed CT scans by the two configurations and precisely aligned resultant 3D images so that the same position in the specimen is compared. We could extract the osteocyte canaliculi and evaluate the mineral density of their surroundings. The degree of mineralization varied for each osteocyte lacuna and canaliculus. The multi-scan microscopic X-ray CT is a powerful tool for analyzing bone mineralization.

Original languageEnglish
Title of host publicationInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings
Pages199-204
Number of pages6
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes
EventInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings, XNPIG 2012 - Tokyo, Japan
Duration: 2012 Mar 52012 Mar 7

Publication series

NameAIP Conference Proceedings
Volume1466
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

OtherInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings, XNPIG 2012
CountryJapan
CityTokyo
Period12/3/512/3/7

Keywords

  • Phase contrast
  • Synchrotron Radiation
  • Talbot interferometry
  • X-ray microscope

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Nango, N., Kubota, S., Yashiro, W., Momose, A., Takada, Y., & Matsuo, K. (2012). Constructing a multi-scan synchrotron X-ray microscope to study the function of osteocyte canaliculi in mouse bone. In International Workshop on X-Ray and Neutron Phase Imaging with Gratings (pp. 199-204). (AIP Conference Proceedings; Vol. 1466). https://doi.org/10.1063/1.4742292