Constitutive expression of small heat shock protein in an htpG disruptant of the cyanobacterium Synechococcus sp. PCC 7942

Kouji Kojima, Hitoshi Nakamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In cyanobacteria, a disruptant of hspA encoding a small heat shock protein homologue, shows decreased cell growth rates at moderately high temperatures, and loss of both basal and acquired thermo-tolerances, which resemble the phenotype of an htpG disruptant. In vitro studies have shown that both small heat shock protein and Hsp90 can bind and keep non-native proteins in a refolding-competent state under denaturing conditions. The aim of the present study is to elucidate whether constitutive expression of HspA can functionally replace HtpG, a prokaryotic homolog of Hsp90, in the cyanobacterium Synechococcus sp. PCC 7942. HspA did not improve the viability of the htpG disruptant at a lethal temperature, although it did that of the wild type. It did not improve an iron-starved phenotype of the mutant under normal growth conditions, a novel phenotype found in the present study. These results suggest that cellular function of HtpG may differ significantly from that of HspA.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalCurrent Microbiology
Volume50
Issue number5
DOIs
Publication statusPublished - 2005 May 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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