Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants

Arata Suenaga, Kanami Moriya, Yutaka Sonoda, Akira Ikeda, Nicolaus Von Wirén, Toshihiko Hayakawa, Junji Yamaguchi, Tomoyuki Yamaya

    Research output: Contribution to journalArticlepeer-review

    138 Citations (Scopus)

    Abstract

    To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

    Original languageEnglish
    Pages (from-to)206-211
    Number of pages6
    JournalPlant and Cell Physiology
    Volume44
    Issue number2
    DOIs
    Publication statusPublished - 2003 Feb 1

    Keywords

    • AMT2
    • Ammonium transporter
    • Antibody
    • Functional expression
    • Rice

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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