Consistent focal cerebral ischemia without posterior cerebral artery occlusion and its real-time monitoring in an intraluminal suture model in mice: Laboratory investigation

Yosuke Akamatsu, Hiroaki Shimizu, Atsushi Saito, Miki Fujimura, Teiji Tominaga

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Object. In the intraluminal suture model of middle cerebral artery occlusion (MCAO) in the mouse, disturbance of blood flow from the internal carotid artery to the posterior cerebral artery (PCA) may affect the size of the infarction. In this study, PCA involvement in the model was investigated and modified for consistent MCAO without involving the PCA territory. Methods. Thirty-seven C57Bl/6 mice were randomly divided into 4 groups according to the length of coating over the tip of the suture (1, 2, 3, or 4 mm) and subjected to transient MCAO for 2 hours. Real-time topographical cerebral blood flow was monitored over both hemispheres by laser speckle flowmetry. After 24 hours of reperfusion, the infarct territories and volumes were evaluated. Results. The 1- and 2-mm coating groups showed all lesions in the MCA territory. In the 3- and 4-mm coating groups, 62.5% and 75% of mice, respectively, showed lesions in both the MCA and the PCA territories and other lesions in the MCA territory. Mice in the 1- and 2-mm coating groups had significantly smaller infarct volumes than the 3- and 4-mm groups. Laser speckle flowmetry was useful to distinguish whether the PCA territory would undergo infarction. Conclusions. Small changes in the coating length of the intraluminal suture may be critical, and 1-2 mm of coating appeared to be optimal to produce consistent MCAO without involving the PCA territory. Laser speckle flowmetry could predict the territory of infarction and improve the consistency of the infarct size.

Original languageEnglish
Pages (from-to)657-664
Number of pages8
JournalJournal of neurosurgery
Volume116
Issue number3
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • Cerebral ischemia
  • Laser speckle flowmetry
  • Middle cerebral artery
  • Mouse
  • Posterior cerebral artery
  • Suture
  • Vascular disorders

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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