Conserved smooth muscle contractility and blood pressure increase in response to high-salt diet in mice lacking the β3 subunit of the voltage-dependent calcium channel

Manabu Murakami, Hisao Yamamura, Agnieszka Murakami, Tadashi Okamura, Kazuo Nunoki, Minori Mitui-Saito, Katsuhiko Muraki, Takuzou Hano, Yuji Imaizumi, Veit Flockerzi, Teruyuki Yanagisawa, Shigeru Motomura, Teruhiko Toyo-Oka, Yasunobu Hirata

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Voltage-dependent calcium channels are crucially important for calcium influx and the following smooth muscle contraction. β subunits of these channels are known to modify calcium currents through pore-forming α1 subunits. Among the four reported independent β subunits, the β3 subunit is expressed in smooth muscle cells and thought to compose L-type calcium channels in the tissue. To determine the role of the β3 subunit in the cardiovascular system, we have analyzed β3-null mice. Electrophysiological examinations proved the existence of dihydropyridine (DHP)-sensitive, L-type calcium channels in the smooth muscle cells. β3-null mice show no apparent changes in smooth muscle contraction and sensitivity to DHP, and normal blood pressure when they are raised on a normal diet, but the β3 subunit deficient mice show elevated blood pressure in response to a high-salt diet, with significant reductions in plasma catecholamine concentrations. Our finding strongly suggests a close relationship between voltage-dependent channels and high blood pressure.

Original languageEnglish
Pages (from-to)S69-S73
JournalJournal of cardiovascular pharmacology
Volume36
Issue number6 SUPPL. 2
DOIs
Publication statusPublished - 2000

Keywords

  • Blood pressure
  • Calcium channel
  • Smooth muscle
  • β-subunits

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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    Murakami, M., Yamamura, H., Murakami, A., Okamura, T., Nunoki, K., Mitui-Saito, M., Muraki, K., Hano, T., Imaizumi, Y., Flockerzi, V., Yanagisawa, T., Motomura, S., Toyo-Oka, T., & Hirata, Y. (2000). Conserved smooth muscle contractility and blood pressure increase in response to high-salt diet in mice lacking the β3 subunit of the voltage-dependent calcium channel. Journal of cardiovascular pharmacology, 36(6 SUPPL. 2), S69-S73. https://doi.org/10.1097/00005344-200000006-00015