Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi, Erina Yazaki, Kazuhei Kudo, Toshifumi Inada

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

Original languageEnglish
Pages (from-to)3254-3263
Number of pages10
JournalFEBS Letters
Volume590
Issue number18
DOIs
Publication statusPublished - 2016 Sep 1

Keywords

  • Pelota
  • mRNA quality control
  • nonstop decay
  • stalled ribosomes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Conserved functions of human Pelota in mRNA quality control of nonstop mRNA'. Together they form a unique fingerprint.

Cite this