Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi; Erina Yazaki; Kazuhei Kudo; Toshifumi Inada

doi:10.1002/1873-3468.12366

Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi, Erina Yazaki, Kazuhei Kudo, Toshifumi Inada

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

Original language	English
Pages (from-to)	3254-3263
Number of pages	10
Journal	FEBS Letters
Volume	590
Issue number	18
DOIs	https://doi.org/10.1002/1873-3468.12366
Publication status	Published - 2016 Sep 1

Keywords

Pelota
mRNA quality control
nonstop decay
stalled ribosomes

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1002/1873-3468.12366

Fingerprint Dive into the research topics of 'Conserved functions of human Pelota in mRNA quality control of nonstop mRNA'. Together they form a unique fingerprint.

Cite this

@article{31e2536933ae47e690a7c3ad96633c7f,

title = "Conserved functions of human Pelota in mRNA quality control of nonstop mRNA",

abstract = "Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.",

keywords = "Pelota, mRNA quality control, nonstop decay, stalled ribosomes",

author = "Ken Ikeuchi and Erina Yazaki and Kazuhei Kudo and Toshifumi Inada",

year = "2016",

month = sep,

day = "1",

doi = "10.1002/1873-3468.12366",

language = "English",

volume = "590",

pages = "3254--3263",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "18",

}

TY - JOUR

T1 - Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

AU - Ikeuchi, Ken

AU - Yazaki, Erina

AU - Kudo, Kazuhei

AU - Inada, Toshifumi

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

AB - Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

KW - Pelota

KW - mRNA quality control

KW - nonstop decay

KW - stalled ribosomes

UR - http://www.scopus.com/inward/record.url?scp=84986325569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986325569&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.12366

DO - 10.1002/1873-3468.12366

M3 - Article

C2 - 27543824

AN - SCOPUS:84986325569

VL - 590

SP - 3254

EP - 3263

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 18

ER -

Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this