Abstract
Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.
Original language | English |
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Pages (from-to) | 3254-3263 |
Number of pages | 10 |
Journal | FEBS Letters |
Volume | 590 |
Issue number | 18 |
DOIs | |
Publication status | Published - 2016 Sep 1 |
Externally published | Yes |
Keywords
- Pelota
- mRNA quality control
- nonstop decay
- stalled ribosomes
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology