Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi; Erina Yazaki; Kazuhei Kudo; Toshifumi Inada

doi:10.1002/1873-3468.12366

Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Ken Ikeuchi, Erina Yazaki, Kazuhei Kudo, Toshifumi Inada

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

Original language	English
Pages (from-to)	3254-3263
Number of pages	10
Journal	FEBS Letters
Volume	590
Issue number	18
DOIs	https://doi.org/10.1002/1873-3468.12366
Publication status	Published - 2016 Sep 1
Externally published	Yes

Keywords

Pelota
mRNA quality control
nonstop decay
stalled ribosomes

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1002/1873-3468.12366

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title = "Conserved functions of human Pelota in mRNA quality control of nonstop mRNA",

abstract = "Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.",

keywords = "Pelota, mRNA quality control, nonstop decay, stalled ribosomes",

author = "Ken Ikeuchi and Erina Yazaki and Kazuhei Kudo and Toshifumi Inada",

note = "Publisher Copyright: {\textcopyright} 2016 Federation of European Biochemical Societies",

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doi = "10.1002/1873-3468.12366",

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T1 - Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

AU - Ikeuchi, Ken

AU - Yazaki, Erina

AU - Kudo, Kazuhei

AU - Inada, Toshifumi

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

AB - Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3′ end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3′ end of mRNA in dom34∆ mutant cells.

KW - Pelota

KW - mRNA quality control

KW - nonstop decay

KW - stalled ribosomes

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ER -

Conserved functions of human Pelota in mRNA quality control of nonstop mRNA

Abstract

Keywords

ASJC Scopus subject areas

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