Conserved and divergent expression patterns of markers of axial development in the laboratory opossum, Monodelphis domestica

Michio Yoshida, Eriko Kajikawa, Daisuke Yamamoto, Daisuke Kurokawa, Shigenobu Yonemura, Kensaku Kobayashi, Hiroshi Kiyonari, Shinichi Aizawa

    Research output: Contribution to journalArticle

    Abstract

    Background: Previous comparative studies suggest that the requirement for Nodal in epiblast and hypoblast development is unique to mammalians. Expression of anterior visceral endoderm (AVE) genes in the visceral endoderm and of their orthologs in the hypoblast may be unique to mammalians and avians, and is absent in the reptilian hypoblast. Axis formation in reptiles is signaled by the formation of the posterior marginal epiblast (PME), which expresses a series of primitive streak genes. To assess the phylogenetic origin of Nodal and AVE gene expression and axis formation in amniotes, we examined marker gene expression in gray short-tailed opossum, a metatherian. Results: Nodal was expressed in neither epiblast nor hypoblast of opossum embryos. No AVE genes were expressed in the opossum hypoblast. Attainment of polarity in the embryonic disk was signaled by Nodal, Wnt3a, Fgf8, and Bra expression in the PME at 8.5 days post-coitus. Conclusions: Nodal expression in epiblast or hypoblast may be unique to eutherians. AVE gene expression in visceral endoderm and hypoblast may have been independently acquired in eutherian and avian lineages. PME formation appears to be the event that signals axis formation in reptilian and metatherian embryos, and thus may be an ancestral characteristic of basal amniotes. Developmental Dynamics 245:1176–1188, 2016.

    Original languageEnglish
    Pages (from-to)1176-1188
    Number of pages13
    JournalDevelopmental Dynamics
    Volume245
    Issue number12
    DOIs
    Publication statusPublished - 2016 Dec 1

    Keywords

    • AVE gene
    • Nodal
    • axis formation
    • hypoblast
    • metatherian
    • posterior marginal epiblast (PME)

    ASJC Scopus subject areas

    • Developmental Biology

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  • Cite this

    Yoshida, M., Kajikawa, E., Yamamoto, D., Kurokawa, D., Yonemura, S., Kobayashi, K., Kiyonari, H., & Aizawa, S. (2016). Conserved and divergent expression patterns of markers of axial development in the laboratory opossum, Monodelphis domestica. Developmental Dynamics, 245(12), 1176-1188. https://doi.org/10.1002/dvdy.24459