Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA

Yusuke Sato; Yuki Takahashi; Takaaki Tanabe; Seiichi Nishizawa

doi:10.1039/d0ob00794c

Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA

Yusuke Sato, Yuki Takahashi, Takaaki Tanabe, Seiichi Nishizawa

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3′-overhang and double-stranded sequences of target siRNAs.

Original language	English
Pages (from-to)	4009-4013
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	18
Issue number	21
DOIs	https://doi.org/10.1039/d0ob00794c
Publication status	Published - 2020 Jun 7

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1039/d0ob00794c

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abstract = "We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3′-overhang and double-stranded sequences of target siRNAs.",

author = "Yusuke Sato and Yuki Takahashi and Takaaki Tanabe and Seiichi Nishizawa",

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AU - Nishizawa, Seiichi

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