Confirmation of D-aspartic acid in the novel dipeptide beta-aspartylglycine isolated from tissue extract of Aplysia kurodai.

M. Sato, T. Yamaguchi, N. Kanno, Y. Sato

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    A novel o-phthalaldehyde-reactive compound was found in the h.p.l.c. chromatogram of Aplysia kurodai extract. This compound was isolated by ion-exchange chromatography and preparative high-voltage paper electrophoresis. It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine. This compound resisted cleavage in the Edman reaction. This peptide was inferred to be beta-D-aspartylglycine, and this was confirmed by synthesis. beta-D-Aspartylglycine was detected in all tissues of Aplysia kurodai, with especially high concentrations in body wall (skin and muscle) and gill.

    Original languageEnglish
    Pages (from-to)617-620
    Number of pages4
    JournalThe Biochemical journal
    Volume263
    Issue number2
    DOIs
    Publication statusPublished - 1989 Oct 15

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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