Conditional knockout of Lgr4 leads to impaired ductal elongation and branching morphogenesis in mouse mammary glands

K. Oyama, Y. Mohri, M. Sone, A. Nawa, K. Nishimori

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have analyzed the function of LGR4 in the development of various mouse epithelial tissues. Here we first report the retarded invasion of mammary ducts into the fat pad observed in Lgr4 K5 KO mice at 4 weeks, compared with that of age-matched Lgr4 K5 ctrl. Furthermore, we demonstrate a significant decrease in mammary ductal branching in Lgr4 K5 KO at several stages (4, 6 and 8 weeks). On the other hand, immunohistochemical analysis of the mammary gland of Lgr4 K5 KO using anti-αSMA, anti-K18 and anti-laminin antibodies showed structures similar to those of Lgr4 K5 ctrl mammary glands. In addition, we did not detect significant differences in the expression of ERα, which was suggested to be a downstream molecule of LGR4, and Lgr4 K5 KO showed no retarded invasion in the response to 17β-estradiol administration. Furthermore, the phosphorylated form of Smad1/5/8 was normally detected in the mammary gland of Lgr4 K5 KO.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalSexual Development
Volume5
Issue number4
DOIs
Publication statusPublished - 2011 Jul

Keywords

  • Knockout mouse
  • Lgr4
  • Mammary gland
  • Morphogenesis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Embryology
  • Developmental Biology

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