Conditional activation of RhoA suppresses the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation

Toshimitsu Fuse, Yoshiakira Kanai, Masami Kanai-Azuma, Misao Suzuki, Kazuhiro Nakamura, Hisashi Mori, Yoshihiro Hayashi, Masayoshi Mishina

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Gastrulation is a pivotal event of mouse early embryogenesis. In telencephalin (TLCN)-Cre mice carrying the Cre recombinase gene inserted into the translational initiation site of the TLCN gene, Cre-mediated recombination took place at the postimplantation stage. To examine the role of RhoA signaling in early embryogenesis, we produced Rho36 mice carrying constitutively active RhoAG14V gene inducible by Cre recombinase and crossed with TLCN-Cre mice. In doubly transgenic embryos at the gastrulation stage, there appeared an abnormal bulge of cells protruded from the primitive streak region into the amniotic cavity. The bulged cell mass expressed the epiblast marker gene Oct3 and E-cadherin, but not the primitive streak marker gene T except for the basal portion. These results suggest that the conditional activation of RhoA signaling suppressed the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalBiochemical and biophysical research communications
Volume318
Issue number3
DOIs
Publication statusPublished - 2004 Jun 4

Keywords

  • Cre recombinase
  • E-cadherin
  • Epithelial-mesenchymal transition
  • Gastrulation
  • Mouse embryogenesis
  • Rho

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Conditional activation of RhoA suppresses the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation'. Together they form a unique fingerprint.

Cite this