Concurrent observations of astrocytic Ca2+ activity and multisite extracellular potentials from an intact cerebral cortex

Jorge Riera, Takeshi Ogawa, Rieko Hatanaka, Takakuni Goto, Akira Sumiyoshi, Herve Enjieu Kadji, Sakura Nakauchi, Ryuta Kawashima

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

In basic neuroscience, the attention has been recently focused on the role played by the protoplasmic astrocytes in modulating the activity of nearby neurons or else on assisting a long-term/sustained communication between these neurons and the surrounding microvasculature. However, to understand the physiological mechanisms underlying such a multiscale interactions in space and time, novel methodologies are required. This paper reports about an experimental setting and a procedure that was developed to obtain concurrently twophoton astrocytic Ca2 imaging and multisite large-scale extracellular potentials as recorded by a silicon-based probe. Solutions to several technical drawbacks (e.g. removal of photoelectric artifacts, the establishment of safety ranges for microinjection) are provided which are intrinsic to the technology and procedure utilized. Through the use of SR101 to stain protoplasmic astrocytes, it was possible to combine functional information represented by the Ca2 activity in individual astrocytes and the LFPs with geometrical descriptors of the astrocytic/ vessel networks. Spatial distributions of neurons (Nissl fluorescent - yellow), astrocytes (sulforhodamine 101 - green) and vessels (FITC-dextran - red) in a coronal section of the barrel cortex. This postmortem brain section was obtained from a perfused brain following the in vivo experiment.

Original languageEnglish
Pages (from-to)147-160
Number of pages14
JournalJournal of Biophotonics
Volume3
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Keywords

  • LFP/MUA
  • Multiscale cellular activity
  • Neuronal modulation
  • Neurovascular coupling
  • Two-photon laser scanning microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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