TY - JOUR
T1 - Concurrent connection of embryonic chick heart using a microfluidic device for Organ-Explant-Chip
AU - Owaki, Hirofumi
AU - Masuda, Taisuke
AU - Kawahara, Tomohiro
AU - Miyasaka, Kota
AU - Ogura, Toshihiko
AU - Arai, Fumihito
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research on Innovative Areas "Bio Assembler" (23106002, 24106506) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Nagoya University Global COE Program, -Nano
PY - 2013
Y1 - 2013
N2 - We propose a concurrent microvascular connection method called suction-induced vascular fixation (SVF) method for the achievement of Organ-Explant-Chip which is a biologically-designed simulator having biological materials such as cells, tissues, and organs. The advantages of proposed method with using a microfluidic device are as follows: (1) operation of flexible objects (blood vessels), (2) alignment the blood vessels concurrently, and (3) reduction of the DOFs of the blood vessels. From the experimental results, we confirmed that four cardiovascular of the explanted embryonic chick heart can be induced into the fabricated microfluidic device concurrently. We have also succeeded in construction of hybrid circulatory system between artifacts and embryonic chick heart, and monitoring the response of the heart of chick embryo by supplying the culture medium.
AB - We propose a concurrent microvascular connection method called suction-induced vascular fixation (SVF) method for the achievement of Organ-Explant-Chip which is a biologically-designed simulator having biological materials such as cells, tissues, and organs. The advantages of proposed method with using a microfluidic device are as follows: (1) operation of flexible objects (blood vessels), (2) alignment the blood vessels concurrently, and (3) reduction of the DOFs of the blood vessels. From the experimental results, we confirmed that four cardiovascular of the explanted embryonic chick heart can be induced into the fabricated microfluidic device concurrently. We have also succeeded in construction of hybrid circulatory system between artifacts and embryonic chick heart, and monitoring the response of the heart of chick embryo by supplying the culture medium.
KW - Bionic simulator
KW - Chick embryo
KW - Microfluidic device
KW - Microvascular connection
UR - http://www.scopus.com/inward/record.url?scp=84883882741&partnerID=8YFLogxK
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U2 - 10.1016/j.procir.2013.01.041
DO - 10.1016/j.procir.2013.01.041
M3 - Conference article
AN - SCOPUS:84883882741
VL - 5
SP - 205
EP - 209
JO - Procedia CIRP
JF - Procedia CIRP
SN - 2212-8271
T2 - 1st CIRP Conference on BioManufacturing, BioM 2013
Y2 - 3 March 2013 through 5 March 2013
ER -