Concomitant enhancement of the response to Mls-1a antigens and the induction of post-thymectomy autoimmune gastritis in BALB/c mice

K. Murakami, M. Hosono, H. Murayama, Y. Mori, A. Nishio, M. Fukumoto, Y. Watanabe, M. Inaba, K. Kuribayashi, M. Sakai, T. Masuda

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8 Citations (Scopus)

Abstract

We examined the role of Mls antigens in the induction of autoimmune gastritis (AIG) in BALB/c and DBA/2 mice subjected to thymectomy. The prevalence of AIG in Mls-1b mice which underwent thymectomy on day 3 after birth (3d-Tx) was 78% (mean), while in Mls-1a DBA/2 mice it was < 6%. Whereas AIG-negative 3d-Tx DBA/2 mice produced 2-mercaptoethanol (2-ME)-sensitive antiparietal cell autoantibody, AIG-positive BALB/c mice made 2-ME-resistant anti-parietal cell autoantibody. In addition, the prevalence of AIG in 3d-Tx BALB/c mice which were rendered tolerant to Mls-1a antigens by injection of bone marrow cells from (BALB/c x DBA/2)F1 mice within 24 h after birth was decreased compared with the non-tolerant control mice; the prevalence being 80% in the controls and 30% in the tolerant animals. Thus, the activation of helper T cells, including T cells responding to Mls-1a antigens and including immunoglobulin class switch, appeared to be closely associated with the induction of AIG. Flow cytometric analysis confirmed that CD4-Vβ6 T cells had increased in the regional lymph nodes of the stomach in AIG mice. However, an increase in the number of Vβ11 T cells, which are known to increase in 3d-Tx mice, occurred in the CD8, but not in the CD4 T cell population. Injection of MoAb to L3T4, but not Lyt2, Vβ6- or Vβ8-TCR, into 3d-Tx BALB/c and syngeneic nude mice which had received spleen cells of 3d-Tx BALB/c mice bearing AIG completely abrogated the development of AIG, despite there being remarkable decreases in T cells expressing relevant markers to the injected antibodies in all the mice. These findings suggest that the increase of Vβ6+ L3T4+ T cells in AIG mice was concomitant with the activation of AIG-inducing Vβ6- L3T4+ T cells.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalClinical and Experimental Immunology
Volume92
Issue number3
DOIs
Publication statusPublished - 1993

Keywords

  • Mls antigen
  • autoantibody
  • autoimmune disease
  • gastritis
  • thymectomy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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