Conalbumin-conjugated silica gel, a new chiral stationary phase for high-performance liquid chromatogaphy

Nariyasu Mano, Yoshiya Oda, Toshinobu Miwa, Naoki Asakawa, Yutaka Yoshida, Tadashi Sato

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A new chiral stationary phase using conalbumin (from chicken egg white) was developed for high-performance liquid chromatography. Chiral resolution of racemic azelastine, an antiallergic drug, was achieved on a conalbumin-conjugated silica gel column. The effects of the pH, the concentration of organic solvents and salts in the mobile phase, and the temperature on the capacity factor and resolution of racemic azelastine were examined. This column shows good stability and can separate optical isomers with an aqueous mobile phase. It should be very useful in studies on pharmacokinetics and in clinical chemistry.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalJournal of Chromatography A
Volume603
Issue number1-2
DOIs
Publication statusPublished - 1992 Jun 19
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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