Compressive force induces prostaglandin E2 production via cyclooxygenase-2 in synovial cell

Hisashi Ichimiya; Tetsu Takahashi; Wataru Ariyoshi; Toshinori Okinaga; Tatsuji Nishihara

doi:10.1016/j.ajoms.2012.01.006

Compressive force induces prostaglandin E₂ production via cyclooxygenase-2 in synovial cell

Hisashi Ichimiya, Tetsu Takahashi, Wataru Ariyoshi, Toshinori Okinaga, Tatsuji Nishihara

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Objectives: Prostaglandin E₂ (PGE₂) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE₂ and cyclooxygenase (COX). Materials and methods: Rabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE₂ release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction. Results: Compressive force was found to enhanced the production of PGE₂ andmRNAexpression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE₂. Conclusions: Our results indicated that the compressed force enhanced PGE₂ production through COX-2 expression in synovial cells.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/j.ajoms.2012.01.006
Publication status	Published - 2012 May
Externally published	Yes

Keywords

Compressive force
Cyclooxygenase-2
Synovial fibroblast

ASJC Scopus subject areas

Surgery
Oral Surgery
Pathology and Forensic Medicine
Otorhinolaryngology

Access to Document

10.1016/j.ajoms.2012.01.006

Cite this

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title = "Compressive force induces prostaglandin E2 production via cyclooxygenase-2 in synovial cell",

abstract = "Objectives: Prostaglandin E2 (PGE2) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE2 and cyclooxygenase (COX). Materials and methods: Rabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE2 release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction. Results: Compressive force was found to enhanced the production of PGE2 andmRNAexpression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE2. Conclusions: Our results indicated that the compressed force enhanced PGE2 production through COX-2 expression in synovial cells.",

keywords = "Compressive force, Cyclooxygenase-2, Synovial fibroblast",

author = "Hisashi Ichimiya and Tetsu Takahashi and Wataru Ariyoshi and Toshinori Okinaga and Tatsuji Nishihara",

year = "2012",

month = may,

doi = "10.1016/j.ajoms.2012.01.006",

language = "English",

volume = "24",

pages = "115--118",

journal = "Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology",

issn = "2212-5558",

publisher = "Elsevier Limited",

number = "2",

}

TY - JOUR

T1 - Compressive force induces prostaglandin E2 production via cyclooxygenase-2 in synovial cell

AU - Ichimiya, Hisashi

AU - Takahashi, Tetsu

AU - Ariyoshi, Wataru

AU - Okinaga, Toshinori

AU - Nishihara, Tatsuji

PY - 2012/5

Y1 - 2012/5

N2 - Objectives: Prostaglandin E2 (PGE2) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE2 and cyclooxygenase (COX). Materials and methods: Rabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE2 release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction. Results: Compressive force was found to enhanced the production of PGE2 andmRNAexpression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE2. Conclusions: Our results indicated that the compressed force enhanced PGE2 production through COX-2 expression in synovial cells.

AB - Objectives: Prostaglandin E2 (PGE2) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE2 and cyclooxygenase (COX). Materials and methods: Rabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE2 release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction. Results: Compressive force was found to enhanced the production of PGE2 andmRNAexpression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE2. Conclusions: Our results indicated that the compressed force enhanced PGE2 production through COX-2 expression in synovial cells.

KW - Compressive force

KW - Cyclooxygenase-2

KW - Synovial fibroblast

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