Compressive force induces prostaglandin E2 production via cyclooxygenase-2 in synovial cell

Hisashi Ichimiya, Tetsu Takahashi, Wataru Ariyoshi, Toshinori Okinaga, Tatsuji Nishihara

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objectives: Prostaglandin E2 (PGE2) and inflammatory cytokines play pivotal roles in the pathogenesis of temporomandibular joint (TMJ) diseases. These mediators are influenced by mechanical loading. To clarify a reaction to mechanical stress of synovial cells, we examined the effects of compressive force on levels of expression of PGE2 and cyclooxygenase (COX). Materials and methods: Rabbit synovial lineage cell (HIG-82) were continuously compressed using a conventional method. PGE2 release in conditioned medium was monitored by enzyme linked immunosorbent assay. The expression of COX mRNA in synovial cells was analyzed by reverse transcriptase-polymerase chain reaction. Results: Compressive force was found to enhanced the production of PGE2 andmRNAexpression of COX-2 in synovial cells. Further, indomethacin blocked compressive force-mediated production of PGE2. Conclusions: Our results indicated that the compressed force enhanced PGE2 production through COX-2 expression in synovial cells.

Original languageEnglish
Pages (from-to)115-118
Number of pages4
JournalJournal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Issue number2
Publication statusPublished - 2012 May
Externally publishedYes


  • Compressive force
  • Cyclooxygenase-2
  • Synovial fibroblast

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Otorhinolaryngology


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