Comprehensive analysis, with an in-house cDNA microarray, of gene expression in the diabetic mouse retina

Nanami Uehara-Adachi, Atsushi Mizota, Emiko Adachi-Usami, Shuichi Yamamoto, Akinori Ishihara, Katsuro Iwase, Satoko Ohtsuka, Masaki Takiguchi, Masaki Kato, Yoshinori Nimura, Naohiko Seki, Hiroki Kodama

Research output: Contribution to journalArticle

Abstract

Purpose: To clarify the molecular pathophysiology of diabetic retinopathy (DR), we performed comprehensive gene expression analysis of the mouse retina under diabetic conditions with an in-house cDNA microarray. Methods: We prepared a microarray harboring 2,880 cDNAs derived from a mouse retina. Amplified cRNAs of diabetic and age-matched control mouse retinas at 1 and 3 months after intraperitoneal injection of streptozotocin were subjected to array analysis. Results: Genes significantly up-regulated in the diabetic state were listed, and they included a number of genes for oxidative phosphorylation in mitochondoria. Conclusions: Our finding of up-regulation of genes for oxidative phosphorylation in the diabetic retina differs from recently reported findings of down-regulation of these genes in the skeletal muscle of diabetic mice. The diabetic retina seems to be in a potentially activated state for glucose metabolism, presumably because of an increase in insulin-independent glucose influx.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalFolia Ophthalmologica Japonica
Volume56
Issue number2
Publication statusPublished - 2005 Feb

Keywords

  • Diabetes Mellitus
  • Diabetic Retinopathy
  • In-House Microarray
  • Oxidative Phosphorylation
  • Streptozotocin

ASJC Scopus subject areas

  • Ophthalmology

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    Uehara-Adachi, N., Mizota, A., Adachi-Usami, E., Yamamoto, S., Ishihara, A., Iwase, K., Ohtsuka, S., Takiguchi, M., Kato, M., Nimura, Y., Seki, N., & Kodama, H. (2005). Comprehensive analysis, with an in-house cDNA microarray, of gene expression in the diabetic mouse retina. Folia Ophthalmologica Japonica, 56(2), 85-89.