Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena

Masaaki Iwamoto, Hiroko Osakada, Chie Mori, Yasuhiro Fukuda, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ~30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type or physiological condition. Here, we present evidence for compositionally distinct NPCs that form within a single cell in a binucleated ciliate. In Tetrahymena thermophila, each cell contains both a transcriptionally active macronucleus (MAC) and a germline micronucleus (MIC). By combining in silico analysis, mass spectrometry analysis for immunoisolated proteins and subcellular localization analysis of GFP-fused proteins, we identified numerous novel components of MAC and MIC NPCs. Core members of the Nup107-Nup160 scaffold complex were enriched in MIC NPCs. Strikingly, two paralogs of Nup214 and of Nup153 localized exclusively to either the MAC or MIC NPCs. Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively. Our results argue that functional nuclear dimorphism in ciliates is likely to depend on the compositional and structural specificity of NPCs.

Original languageEnglish
Pages (from-to)1822-1834
Number of pages13
JournalJournal of cell science
Volume130
Issue number10
DOIs
Publication statusPublished - 2017 May 15

Keywords

  • FG-Nup
  • Nuclear dimorphism
  • Nuclear envelope
  • Nucleoporin
  • Y-complex

ASJC Scopus subject areas

  • Cell Biology

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