Complexing DNA Origami Frameworks through Sequential Self-Assembly Based on Directed Docking

Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Ordered DNA origami arrays have the potential to compartmentalize space into distinct periodic domains that can incorporate a variety of nanoscale objects. Herein, we used the cavities of a preassembled 2D DNA origami framework to incorporate square-shaped DNA origami structures (SQ-origamis). The framework was self-assembled on a lipid bilayer membrane from cross-shaped DNA origami structures (CR-origamis) and subsequently exposed to the SQ-origamis. High-speed AFM revealed the dynamic adsorption/desorption behavior of the SQ-origamis, which resulted in continuous changing of their arrangements in the framework. These dynamic SQ-origamis were trapped in the cavities by increasing the Mg2+ concentration or by introducing sticky-ended cohesions between extended staples, both from the SQ- and CR-origamis, which enabled the directed docking of the SQ-origamis. Our study offers a platform to create supramolecular structures or systems consisting of multiple DNA origami components.

Original languageEnglish
Pages (from-to)7061-7065
Number of pages5
JournalAngewandte Chemie - International Edition
Volume57
Issue number24
DOIs
Publication statusPublished - 2018 Jun 11

Keywords

  • DNA origami
  • directed assembly
  • high-speed atomic force microscopy
  • lipid bilayers
  • self-assembly

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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