TY - JOUR
T1 - Complete genomic structure of the cultivated rice endophyte azospirillum sp. B510
AU - Kaneko, Takakazu
AU - Minamisawa, Kiwamu
AU - Isawa, Tsuyoshi
AU - Nakatsukasa, Hiroki
AU - Mitsui, Hisayuki
AU - Kawaharada, Yasuyuki
AU - Nakamura, Yasukazu
AU - Watanabe, Akiko
AU - Kawashima, Kumiko
AU - Ono, Akiko
AU - Shimizu, Yoshimi
AU - Takahashi, Chika
AU - Minami, Chiharu
AU - Fujishiro, Tsunakazu
AU - Kohara, Mitsuyo
AU - Katoh, Midori
AU - Nakazaki, Naomi
AU - Nakayama, Shinobu
AU - Yamada, Manabu
AU - Tabata, Satoshi
AU - Sato, Shusei
PY - 2010/2
Y1 - 2010/2
N2 - We determined the nucleotide sequence of the entire genome of a diazotrophic endophyte, Azospirillum sp. B510. Strain B510 is an endophytic bacterium isolated from stems of rice plants (Oryza sativa cv. Nipponbare). The genome of B510 consisted of a single chromosome (3 311 395 bp) and six plasmids, designated as pAB510a (1 455 109 bp), pAB510b (723 779 bp), pAB510c (681 723 bp), pAB510d (628 837 bp), pAB510e (537 299 bp), and pAB510f (261 596 bp). The chromosome bears 2893 potential protein-encoding genes, two sets of rRNA gene clusters (rrns), and 45 tRNA genes representing 37 tRNA species. The genomes of the six plasmids contained a total of 3416 protein-encoding genes, seven sets of rrns, and 34 tRNAs representing 19 tRNA species. Eight genes for plasmid-specific tRNA species are located on either pAB510a or pAB510d. Two out of eight genomic islands are inserted in the plasmids, pAB510b and pAB510e, and one of the islands is inserted into trnfM-CAU in the rrn located on pAB510e. Genes other than the nif gene cluster that are involved in N2 fixation and are homologues of Bradyrhizobium japonicum USDA110 include fixABCX, fixNOQP, fixHIS, fixG, and fixLJK. Three putative plant hormone-related genes encoding tryptophan 2-monooxytenase (iaaM) and indole-3-acetaldehyde hydrolase (iaaH), which are involved in IAA biosynthesis, and ACC deaminase (acdS), which reduces ethylene levels, were identified. Multiple gene-clusters for tripartite ATP-independent periplasmic-transport systems and a diverse set of malic enzymes were identified, suggesting that B510 utilizes C4-dicarboxylate during its symbiotic relationship with the host plant.
AB - We determined the nucleotide sequence of the entire genome of a diazotrophic endophyte, Azospirillum sp. B510. Strain B510 is an endophytic bacterium isolated from stems of rice plants (Oryza sativa cv. Nipponbare). The genome of B510 consisted of a single chromosome (3 311 395 bp) and six plasmids, designated as pAB510a (1 455 109 bp), pAB510b (723 779 bp), pAB510c (681 723 bp), pAB510d (628 837 bp), pAB510e (537 299 bp), and pAB510f (261 596 bp). The chromosome bears 2893 potential protein-encoding genes, two sets of rRNA gene clusters (rrns), and 45 tRNA genes representing 37 tRNA species. The genomes of the six plasmids contained a total of 3416 protein-encoding genes, seven sets of rrns, and 34 tRNAs representing 19 tRNA species. Eight genes for plasmid-specific tRNA species are located on either pAB510a or pAB510d. Two out of eight genomic islands are inserted in the plasmids, pAB510b and pAB510e, and one of the islands is inserted into trnfM-CAU in the rrn located on pAB510e. Genes other than the nif gene cluster that are involved in N2 fixation and are homologues of Bradyrhizobium japonicum USDA110 include fixABCX, fixNOQP, fixHIS, fixG, and fixLJK. Three putative plant hormone-related genes encoding tryptophan 2-monooxytenase (iaaM) and indole-3-acetaldehyde hydrolase (iaaH), which are involved in IAA biosynthesis, and ACC deaminase (acdS), which reduces ethylene levels, were identified. Multiple gene-clusters for tripartite ATP-independent periplasmic-transport systems and a diverse set of malic enzymes were identified, suggesting that B510 utilizes C4-dicarboxylate during its symbiotic relationship with the host plant.
KW - Azospirillum
KW - N2 fixation
KW - endophyte
KW - plant hormone
KW - rice plant
UR - http://www.scopus.com/inward/record.url?scp=77951908137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951908137&partnerID=8YFLogxK
U2 - 10.1093/dnares/dsp026
DO - 10.1093/dnares/dsp026
M3 - Article
C2 - 20047946
AN - SCOPUS:77951908137
VL - 17
SP - 37
EP - 50
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 1
ER -