TY - JOUR
T1 - Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1
AU - Nakamura, Yasukazu
AU - Kaneko, Takakazu
AU - Sato, Shusei
AU - Ikeuchi, Masahiko
AU - Katoh, Hiroshi
AU - Sasamoto, Shigemi
AU - Watanabe, Akiko
AU - Iriguchi, Mayumi
AU - Kawashima, Kumiko
AU - Kimura, Takaharu
AU - Kishida, Yoshie
AU - Kiyokawa, Chiaki
AU - Kohara, Mitsuyo
AU - Matsumoto, Midori
AU - Matsuno, Ai
AU - Nakazaki, Naomi
AU - Shimpo, Sayaka
AU - Sugimoto, Masako
AU - Takeuchi, Chie
AU - Yamada, Manabu
AU - Tabata, Satoshi
PY - 2002
Y1 - 2002
N2 - The entire genome of a thermophilic unicellular cyanobacterium, Thermosynechococcus elongatus BP-1, was sequenced. The genome consisted of a circular chromosome 2,593,857 bp long, and no plasmid was detected. A total of 2475 potential protein-encoding genes, one set of rRNA genes, 42 tRNA genes representing 42 tRNA species and 4 genes for small structural RNAs were assigned to the chromosome by similarity search and computer prediction. The translated products of 56% of the potential protein-encoding genes showed sequence similarity to experimentally identified and predicted proteins of known function, and the products of 34% of these genes showed sequence similarity to the translated products of hypothetical genes. The remaining 10% lacked significant similarity to genes for predicted proteins in the public DNA databases. Sixty-three percent of the T. elongatus genes showed significant sequence similarity to those of both Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120, while 22% of the genes were unique to this species, indicating a high degree of divergence of the gene information among cyanobacterial strains. The lack of genes for typical fatty acid desaturases and the presence of more genes for heat-shock proteins in comparison with other mesophilic cyanobacteria may be genomic features of thermophilic strains. A remarkable feature of the genome is the presence of 28 copies of group II introns, 8 of which contained a presumptive gene for maturase/reverse transcriptase. A trace of genome rearrangement mediated by the group II introns was also observed.
AB - The entire genome of a thermophilic unicellular cyanobacterium, Thermosynechococcus elongatus BP-1, was sequenced. The genome consisted of a circular chromosome 2,593,857 bp long, and no plasmid was detected. A total of 2475 potential protein-encoding genes, one set of rRNA genes, 42 tRNA genes representing 42 tRNA species and 4 genes for small structural RNAs were assigned to the chromosome by similarity search and computer prediction. The translated products of 56% of the potential protein-encoding genes showed sequence similarity to experimentally identified and predicted proteins of known function, and the products of 34% of these genes showed sequence similarity to the translated products of hypothetical genes. The remaining 10% lacked significant similarity to genes for predicted proteins in the public DNA databases. Sixty-three percent of the T. elongatus genes showed significant sequence similarity to those of both Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120, while 22% of the genes were unique to this species, indicating a high degree of divergence of the gene information among cyanobacterial strains. The lack of genes for typical fatty acid desaturases and the presence of more genes for heat-shock proteins in comparison with other mesophilic cyanobacteria may be genomic features of thermophilic strains. A remarkable feature of the genome is the presence of 28 copies of group II introns, 8 of which contained a presumptive gene for maturase/reverse transcriptase. A trace of genome rearrangement mediated by the group II introns was also observed.
KW - Cyanobacterium
KW - Genomic sequencing
KW - Thermophile
KW - Thermosynechococcus elongatus
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U2 - 10.1093/dnares/9.4.123
DO - 10.1093/dnares/9.4.123
M3 - Article
C2 - 12240834
AN - SCOPUS:0036923541
VL - 9
SP - 123
EP - 130
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 4
ER -