Complete amino-acid sequence of the β-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments

Pascale Garnier, Frédéric Ducancel, Tomohisa Ogawa, Jean Claude Boulain, Françoise Goudey-Perrière, Claude Perrière, André Ménez

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A cDNA encoding a subunit of the verrucotoxin (VTX) has been identified from a cDNA library derived from stonefish venom glands. It encodes a polypeptide of 708 amino-acid residues, followed by a 3'-untranslated region of 895 bp long. The ORF contains the complete mature sequence of the β-subunit of the VTX, as inferred from both the presence of an identical N-terminus sequence and 96% homology among the 506 amino terminus residues found in the partial sequence of the β-subunit of the stonustoxin from Synanceia horrida (Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E. and Yuen, R. (1994) Toxicon 32, 1684-1688). Upstream the mature sequence, we noticed the presence of an incomplete peptide of a 13 amino acids, whose unusual primary structure supports the idea of the existence of a propeptide and/or of a new secretion signal.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume1337
Issue number1
DOIs
Publication statusPublished - 1997 Jan 4
Externally publishedYes

Keywords

  • CDNA
  • Stonefish venom
  • Synanceia
  • Toxin
  • Verrucotoxin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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