Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the developing rat

Tetsuji Mori, Ichiro Okano, Kensaku Mizuno, Masaya Tohyama, Akio Wanaka

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We previously isolated two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) from a rat cDNA library. To examine the functions of these genes, we performed in situ hybridization in the developing rat nervous system. LIMK-1 and LIMK-2 mRNAs mostly co-localized during development and are expressed preferentially in the central nervous system during mid-to-late gestation but the signals decreased during the post-natal period. However, differential gene expression was observed in some nuclei in the CNS; LIMK-1 mRNA was intensely expressed in the facial motor nucleus, the hypoglossal nucleus, deep nuclei of the cerebellum and the layers 3, 5 and 6 of the adult cerebral cortex while only LIMK-2 mRNA was preferentially expressed in the some parts of the epithelium. In the nasal cavity, LIMK-1 and LIMK-2 mRNAs were expressed complementarily. Our results suggest that LIMK-1 and LIMK-2 may have different functions in these regions during development.

Original languageEnglish
Pages (from-to)247-254
Number of pages8
JournalMolecular Brain Research
Volume45
Issue number2
DOIs
Publication statusPublished - 1997 May 1
Externally publishedYes

Keywords

  • Developing nervous system
  • Differential gene expression
  • Hybridization histochemistry
  • In situ
  • LIM motif
  • Non-receptor serine/threonine kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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