Comparison of separation methods for tissue-derived extracellular vesicles in the liver, heart, and skeletal muscle

Adam Matejovič, Shohei Wakao, Masaaki Kitada, Yoshihiro Kushida, Mari Dezawa

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation-based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV-related markers, with minimum levels of intracellular organelle-related markers. Some tissue-specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.

Original languageEnglish
Pages (from-to)482-493
Number of pages12
JournalFEBS Open Bio
Volume11
Issue number2
DOIs
Publication statusPublished - 2021 Feb

Keywords

  • exosome
  • extracellular vesicles
  • heart
  • liver
  • muscle
  • tissue
  • ultracentrifugation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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