Comparison of neutrophil infiltration between type 1 and type 2 autoimmune pancreatitis

Toshiyuki Mitsuyama, Kazushige Uchida, Kimi Sumimoto, Yuri Fukui, Tsukasa Ikeura, Toshiro Fukui, Akiyoshi Nishio, Nobuaki Shikata, Yoshiko Uemura, Sohei Satoi, Nobumasa Mizuno, Kenji Notohara, Tooru Shimosegawa, Giuseppe Zamboni, Luca Frulloni, Kazuichi Okazaki

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Background Characteristics of type 2 autoimmune pancreatitis (AIP) is granulocyte epithelial lesions, called idiopathic duct-centric pancreatitis (IDCP). To clarify pathogenesis of IDCP, we investigated mechanism of neutrophil infiltration in type 1 AIP, called lymphoplasmacytic sclerosing pancreatitis (LPSP) and IDCP. Method This study was performed on resected pancreata from patients with alcoholic chronic pancreatitis (ACP, n = 10), LPSP (n = 10) and IDCP (n = 12). The number of neutrophils around the pancreatic ducts was counted. The expression of neutrophils chemoattractants granulocyte chemotactic protein-2 (GCP-2) and interleukin-8 (IL-8) in the pancreatic duct epithelia was examined using immunohistochemistry. The cell staining intensity is scored as negative (0), weak (1), moderate (2) or strong (3). Results The median number of neutrophils around the interlobular pancreatic ducts was significantly higher in IDCP (15.16; interquartile range [IQR]: 9.74-18.41) than in ACP (2.66; IQR: 1.33-4.33) (P < 0.05) and LPSP (3.16; IQR: 2.74-4.57) (P < 0.01). There was no significant difference in the median number of neutrophils around the intralobular pancreatic ducts among ACP (1.16; IQR: 0.33-3.41), LPSP (3.16; IQR: 0.74-5.5) and IDCP (3.00; IQR: 1.08-7.91). The median score of GCP-2 in the interlobular pancreatic duct epithelia was significantly higher in IDCP (1.5; IQR: 0.25-2) than in ACP (0; IQR: 0-0.75) (P < 0.05) and LPSP (0; IQR: 0-0.75) (P < 0.05). There was no significant difference in the median score of IL-8 in the interlobular pancreatic duct epithelia among ACP (0; IQR: 0-0.75), LPSP (1; IQR: 0-1.75) and IDCP (0.5; IQR: 0-1). Conclusions Significantly increased neutrophil infiltration around the interlobular pancreatic duct in IDCP may depend on GCP-2.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalPancreatology
Volume15
Issue number3
DOIs
Publication statusPublished - 2015 May 1

Keywords

  • Chemokines
  • GCP-2
  • IL-8
  • Neutrophils
  • Type 1 AIP
  • Type 2 AIP

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Gastroenterology

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