Comparison of NAD with NADP-dependent Glutamate Dehydrogenase, and CNT with rGO-modified Electrodes, for the Construction of Glutamate Sensors

Katsuhiko Sato, Toshio Kamijo, Shigehiro Takahashi, Takaya Sato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glutamate sensors were prepared using nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP)-dependent glutamate dehydrogenase (GDH). Glutamate was determined from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH. These two types of GDH enzymes were deposited separately on carbon nanotube (CNT) and reduced graphene oxide (rGO)-modified glassy carbon electrodes, in which CNT and rGO were used to improve the oxidation capacity of the electrodes. An anodic peak current in response to 1 mM glutamate of glutamate sensors comprising each of these four combinations were compared under the same conditions. The glutamate response was the highest when NAD-dependent GDH was deposited on the rGO-modified electrode.

Original languageEnglish
Pages (from-to)2237-2240
Number of pages4
JournalElectroanalysis
Volume30
Issue number10
DOIs
Publication statusPublished - 2018 Oct

Keywords

  • Glutamate sensor
  • carbon nanotube
  • glutamate dehydrogenase
  • reduced graphene oxide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Fingerprint Dive into the research topics of 'Comparison of NAD with NADP-dependent Glutamate Dehydrogenase, and CNT with rGO-modified Electrodes, for the Construction of Glutamate Sensors'. Together they form a unique fingerprint.

  • Cite this