Comparison of intratumoral distribution of 99mTc-MIBI and deoxyglucose in mouse breast cancer models

H. Ohira, K. Kubota, N. Ohuchi, Y. Harada, H. Fukuda, S. Satomi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The aims of this study were to evaluate the distribution of 99mTc-methoxyisobutylisonitrile (MIBI) in 3 animal models of breast cancer, the effect of radiotherapy on 99mTc-MIBI uptake, and the relationship between uptake and microvessel density. Methods: We used syngeneic, subcutaneously transplanted FM3A, MM48, and Ehrlich mouse breast cancer. 99mTc-MIBI and FDG were injected intravenously, and tumor uptake was measured 30 min later. Double-tracer macroautoradiography (ARG) images were prepared with 99mTc-MIBI and 2-deoxy-D-[1-14C]-glucose (14C-DG), analyzed quantitatively, and compared with histology. The radiotherapeutic effects of 20 Gy x-ray irradiation were monitored by measuring tumor volume, tumor uptake, and ARG findings using 99mTc-MIBI and FDG in FM3A tumors. Microvessel density was quantified by immunohistochemical staining for CD34 and compared with ARG using 99mTc-MIBI in FM3A tumors. Results: FM3A, MM48, and Ehrlich tumors showed different growth rates and radiosensitivities. Uptake of FDG, but not of 99mTc-MIBI, correlated significantly with growth rates. Compared with 14C-DG, 99mTc-MIBI accumulated more in cancer cells and less in infiltrating fibroblasts and macrophages in all tumor models. Irradiation significantly decreased 99mTc-MIBI uptake, but a rapid increase was noted at recurrence on day 7. Changes in FDG uptake were not significant at recurrence. Microvessel density in tumor tissue correlated significantly with 99mTc-MIBI uptake on ARG. Conclusion: Accumulation of 99mTc-MIBI in cancer cells is preferential and can be used as a sensitive marker to examine the response to radiotherapy. Angiogenesis seems to enhance accumulation of 99mTc-MIBI in tumors. These characteristics may be favorable for tumor imaging using 99mTc-MIBI.

Original languageEnglish
Pages (from-to)1561-1568
Number of pages8
JournalJournal of Nuclear Medicine
Volume41
Issue number9
Publication statusPublished - 2000

Keywords

  • Autoradiography
  • Breast cancer
  • Deoxyglucose
  • Microvessel
  • Tc-methoxyisobutylisonitrile

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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