Comparison of human selenoprotein P determinants in serum between our original methods and commercially available kits

Yoshiro Saito, Hirofumi Misu, Hiroaki Takayama, Shin Ichiro Takashima, Soichiro Usui, Masayuki Takamura, Shuichi Kaneko, Toshinari Takamura, Noriko Noguchi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Selenoprotein P (SeP) is a selenium (Se)-rich extracellular protein. SeP is identified as a hepatokine, causing insulin resistance in type 2 diabetes. Thus, the measurement of SeP in serum has received much attention, and several enzyme-linked immunosorbent assay (ELISA) kits for SeP determination are now commercially available. In the present study, we determined the serum SeP levels by our original ELISA and sol particle homogeneous immunoassay (SPIA) methods and also by commercially available kits, and these determinants were compared. We found a kit-dependent correlation of the determinants with our methods. These results suggest that the selection of kit is critical for comparison with our previous reports and for discussing the relationship between the serum SeP levels and disease condition.

Original languageEnglish
Pages (from-to)828-832
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume41
Issue number5
DOIs
Publication statusPublished - 2018

Keywords

  • Commercially available kit
  • Enzyme-linked immunosorbent assay (ELISA)
  • Monoclonal antibody
  • Selenoprotein P
  • Sol particle homogeneous immunoassay (SPIA)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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