Comparison of gene expression profiles produced by CAGE, illumina microarray and real time RT-PCR.

André Fujita, Masao Nagasaki, Seiya Imoto, Ayumu Saito, Emi Ikeda, Teppei Shimamura, Rui Yamaguchi, Yoshihide Hayashizaki, Satoru Miyano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Several technologies are currently used for gene expression profiling, such as Real Time RT-PCR, microarray and CAGE (Cap Analysis of Gene Expression). CAGE is a recently developed method for constructing transcriptome maps and it has been successfully applied to analyzing gene expressions in diverse biological studies. The principle of CAGE has been developed to address specific issues such as determination of transcriptional starting sites, the study of promoter regions and identification of new transcripts. Here, we present both quantitative and qualitative comparisons among three major gene expression quantification techniques, namely: CAGE, illumina microarray and Real Time RT-PCR, by showing that the quantitative values of each method are not interchangeable, however, each of them has unique characteristics which render all of them essential and complementary. Understanding the advantages and disadvantages of each technology will be useful in selecting the most appropriate technique for a determined purpose.

Original languageEnglish
Pages (from-to)56-68
Number of pages13
JournalGenome informatics. International Conference on Genome Informatics
Volume24
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Medicine(all)

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