Comparison of CCD-equipped laser speckle flowgraphy with hydrogen gas clearance method in the measurement of optic nerve head microcirculation in rabbits

Hiroaki Takahashi, Tetsuya Sugiyama, Hideki Tokushige, Takatoshi Maeno, Toru Nakazawa, Tsunehiko Ikeda, Makoto Araie

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

The aim of this study was to verify the correlation between mean blur rate (MBR) obtained with CCD-equipped laser speckle flowgraphy (LSFG) and capillary blood flow (CBF) obtained by the hydrogen gas clearance method in rabbit optic nerve head (ONH). Using Japanese white rabbits under systemic anesthesia, a hydrogen electrode was inserted an area of the ONH free from superficial capillaries. MBR was measured with LSFG near the hydrogen electrode. CBF and MBR were measured in the range of 32.4-83.5 mL/min/100 g and 3.5-6.0, respectively. MBR and CBF were significantly correlated (r = 0.73, P < 0.01, n = 14). After inhalation of carbon dioxide (CO2) or intravenous administration of endothelin-1 (ET-1), MBR and CBF were changed in the relative range of 0.74-1.27 and 0.76-1.35, respectively. The relative changes in MBR and CBF induced by CO2 and ET-1 were also significantly correlated (r = 0.67, P < 0.01). The current results suggest that MBR may correlate with CBF and also change with CBF, as an index of blood flow in the ONH, linearly.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalExperimental Eye Research
Volume108
DOIs
Publication statusPublished - 2013 Mar

Keywords

  • CBF
  • Capillary blood flow
  • Hydrogen gas clearance method
  • LSFG
  • Laser speckle flowgraphy
  • MBR
  • Mean blur rate
  • ONH
  • Optic nerve head
  • Rabbit

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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