Comparison of Avidin and Ovomucoid as Chiral Selectors for the Resolution of Drug Enantiomers by High-Performance Liquid Chromatography

Yoshiya Oda, Nariyasu Mano, Naoki Asakawa, Yutaka Yoshida, Tadashi Sato, Terumichi Nakagawa

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The effects of the pH, organic modifiers and buffer salt of the mobile phase upon the retention and separatin characteristics of avidin- and ovomucoid-bonded silica columns as proteinous chiral stationary phases for HPLC were investigated. The enantioselectivity was greatly affected by changing the mobile-phase composition, possibly because finduced alterations in the protein conformation. Hydrophobic and Coulombic interactions play major roles in the retention, and there are some similarities between the avidin column and the ovomucoid column. However, the stereoselectivity does not necessarily reflect the retention processes on these columns. No significant decrease in te retention or enantioselectivity was observed, even after the injection of 200 samples.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
Journalanalytical sciences
Volume9
Issue number2
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Keywords

  • High-performance liquid chromatography
  • avidin column
  • chiral separation
  • ovomucoid column

ASJC Scopus subject areas

  • Analytical Chemistry

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