TY - JOUR
T1 - Comparison of articular cartilage images assessed by high-frequency ultrasound microscope and scanning acoustic microscope
AU - Hagiwara, Yoshihiro
AU - Saijo, Yoshifumi
AU - Ando, Akira
AU - Onoda, Yoshito
AU - Suda, Hideaki
AU - Chimoto, Eiichi
AU - Hatori, Kouki
AU - Itoi, Eiji
N1 - Funding Information:
Acknowledgment This research was supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Young Scientists (B).
PY - 2012/1
Y1 - 2012/1
N2 - Purpose: The purpose of this study was to compare images of a newly developed high-frequency ultrasound imaging system (HFUIS) and scanning acoustic microscope (SAM) and to calculate their Pearson product moment correlations with a view to applying HFUIS for clinical use. Methods: Cylindrical cartilage-bone complexes from adult male Sprague-Dawley rats were obtained. The specimens were immersed in normal saline and scanned by HFUIS. Intensity by HFUIS was normalised by reflection from a steel plate at the same distance. After the scanning, specimens were fixed with paraformaldehyde, decalcified and embedded in paraffin. Thinly sliced tissues were prepared for SAM evaluation. After the scanning, three layers of articular cartilage (superficial, middle and deep) were independently evaluated and their relationships calculated. Results: The superficial and deep layers indicated high relative intensity, whereas the middle layer showed nonhomogeneous relative intensity by HFUIS. A high relative intensity by HFUIS and high sound speed area by SAM had strong correlations (Pearson product moment correlation, superficial layer 0.704, middle layer 0.731). Conclusions: HFUIS produced high-resolution images of the articular cartilage and its intensity was strongly correlated with sound speed by SAM.
AB - Purpose: The purpose of this study was to compare images of a newly developed high-frequency ultrasound imaging system (HFUIS) and scanning acoustic microscope (SAM) and to calculate their Pearson product moment correlations with a view to applying HFUIS for clinical use. Methods: Cylindrical cartilage-bone complexes from adult male Sprague-Dawley rats were obtained. The specimens were immersed in normal saline and scanned by HFUIS. Intensity by HFUIS was normalised by reflection from a steel plate at the same distance. After the scanning, specimens were fixed with paraformaldehyde, decalcified and embedded in paraffin. Thinly sliced tissues were prepared for SAM evaluation. After the scanning, three layers of articular cartilage (superficial, middle and deep) were independently evaluated and their relationships calculated. Results: The superficial and deep layers indicated high relative intensity, whereas the middle layer showed nonhomogeneous relative intensity by HFUIS. A high relative intensity by HFUIS and high sound speed area by SAM had strong correlations (Pearson product moment correlation, superficial layer 0.704, middle layer 0.731). Conclusions: HFUIS produced high-resolution images of the articular cartilage and its intensity was strongly correlated with sound speed by SAM.
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U2 - 10.1007/s00264-011-1263-1
DO - 10.1007/s00264-011-1263-1
M3 - Article
C2 - 21567149
AN - SCOPUS:84858794879
SN - 0341-2695
VL - 36
SP - 185
EP - 190
JO - International Orthopaedics
JF - International Orthopaedics
IS - 1
ER -