TY - JOUR
T1 - Comparison of adenosine triphosphate, microbiological load, and residual protein as indicators for assessing the cleanliness of flexible gastrointestinal endoscopes
AU - Fushimi, Ryo
AU - Takashina, Masaki
AU - Yoshikawa, Hideki
AU - Kobayashi, Hiroyoshi
AU - Okubo, Takashi
AU - Nakata, Seizoh
AU - Kaku, Mitsuo
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/2
Y1 - 2013/2
N2 - Background: This study evaluated 3 potential indicators of gastrointestinal endoscope cleanliness: adenosine triphosphate (ATP), microbiological load, and protein. Methods: Before and after cleaning, ATP and microbiological load were determined from swabs of exterior surfaces and rinses of interior suction/accessory channels. Similarly, before and after cleaning, residual protein was determined from rinses of interior suction/accessory channels. Results: Before cleaning, ATP values were 10,417 relative light units (RLU) from the exterior endoscope surface and 30,281 RLU from the suction/accessory channel rinsates. After cleaning, these ATP values were decreased to 82 RLUs and 104 RLUs, a statistically significant difference. A similar trend was observed with microbiological load, but the change in residual protein from before cleaning to after cleaning was not significant. ATP values reliably reflected microbiological colony counts. Conclusions: ATP measurement can provide a reliable, rapid and practical assessment of endoscope cleanliness for routine monitoring in the clinical setting.
AB - Background: This study evaluated 3 potential indicators of gastrointestinal endoscope cleanliness: adenosine triphosphate (ATP), microbiological load, and protein. Methods: Before and after cleaning, ATP and microbiological load were determined from swabs of exterior surfaces and rinses of interior suction/accessory channels. Similarly, before and after cleaning, residual protein was determined from rinses of interior suction/accessory channels. Results: Before cleaning, ATP values were 10,417 relative light units (RLU) from the exterior endoscope surface and 30,281 RLU from the suction/accessory channel rinsates. After cleaning, these ATP values were decreased to 82 RLUs and 104 RLUs, a statistically significant difference. A similar trend was observed with microbiological load, but the change in residual protein from before cleaning to after cleaning was not significant. ATP values reliably reflected microbiological colony counts. Conclusions: ATP measurement can provide a reliable, rapid and practical assessment of endoscope cleanliness for routine monitoring in the clinical setting.
KW - ATP
KW - Cleanliness
KW - Flexible gastrointestinal endoscope
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U2 - 10.1016/j.ajic.2012.02.030
DO - 10.1016/j.ajic.2012.02.030
M3 - Article
C2 - 22906873
AN - SCOPUS:84873093229
VL - 41
SP - 161
EP - 164
JO - American Journal of Infection Control
JF - American Journal of Infection Control
SN - 0196-6553
IS - 2
ER -