TY - JOUR
T1 - Comparative genome sequencing reveals genomic signature of extreme desiccation tolerance in the anhydrobiotic midge
AU - Gusev, Oleg
AU - Suetsugu, Yoshitaka
AU - Cornette, Richard
AU - Kawashima, Takeshi
AU - Logacheva, Maria D.
AU - Kondrashov, Alexey S.
AU - Penin, Aleksey A.
AU - Hatanaka, Rie
AU - Kikuta, Shingo
AU - Shimura, Sachiko
AU - Kanamori, Hiroyuki
AU - Katayose, Yuichi
AU - Matsumoto, Takashi
AU - Shagimardanova, Elena
AU - Alexeev, Dmitry
AU - Govorun, Vadim
AU - Wisecaver, Jennifer
AU - Mikheyev, Alexander
AU - Koyanagi, Ryo
AU - Fujie, Manabu
AU - Nishiyama, Tomoaki
AU - Shigenobu, Shuji
AU - Shibata, Tomoko F.
AU - Golygina, Veronika
AU - Hasebe, Mitsuyasu
AU - Okuda, Takashi
AU - Satoh, Nori
AU - Kikawada, Takahiro
N1 - Funding Information:
We extend our gratitude to the Federal Ministry of Environment of Nigeria for permitting research on P. vanderplanki. We acknowledge Y. Saito and T. Shiratori for their help with rearing and maintenance of midges and larvae, and Y. Sato for preparing sequencing libraries and for other technical assistance with sequencing. A part of sequencing was supported by OIST internal fund to the Marine Genomics Unit, NIAS internal fund and NIBB internal fund. The supercomputing was supported by the IT Section of OIST and NIAS. A part of computation was performed on the Supercomputer System in National Institute of Genetics. This work was supported in part by Grants-in-Aids from MEXT/JSPS KAKENHI (Grant Number 21688004, 22128001, 23128512, 23780055, 24120006, 25128714 and 25252060), Japan; subsidy of the Russian Government to support the Program of competitive growth of Kazan Federal University among world class academic centres and universities; Russian Foundation for Basic Research (No 12-08-33157 mol_a_ved and No 14-04-01657_A); grant 11.G34.31.0008 from the Ministry of Education and Science of the Russian Federation; RFBR and Tatarstan government grant No 12-04-9707.
Publisher Copyright:
© 2014 Macmillan Publishers Limited. All rights reserved.
PY - 2014/9/12
Y1 - 2014/9/12
N2 - Anhydrobiosis represents an extreme example of tolerance adaptation to water loss, where an organism can survive in an ametabolic state until water returns. Here we report the first comparative analysis examining the genomic background of extreme desiccation tolerance, which is exclusively found in larvae of the only anhydrobiotic insect, Polypedilum vanderplanki. We compare the genomes of P. vanderplanki and a congeneric desiccation-sensitive midge P. nubifer. We determine that the genome of the anhydrobiotic species specifically contains clusters of multi-copy genes with products that act as molecular shields. In addition, the genome possesses several groups of genes with high similarity to known protective proteins. However, these genes are located in distinct paralogous clusters in the genome apart from the classical orthologues of the corresponding genes shared by both chironomids and other insects. The transcripts of these clustered paralogues contribute to a large majority of the mRNA pool in the desiccating larvae and most likely define successful anhydrobiosis. Comparison of expression patterns of orthologues between two chironomid species provides evidence for the existence of desiccation-specific gene expression systems in P. vanderplanki.
AB - Anhydrobiosis represents an extreme example of tolerance adaptation to water loss, where an organism can survive in an ametabolic state until water returns. Here we report the first comparative analysis examining the genomic background of extreme desiccation tolerance, which is exclusively found in larvae of the only anhydrobiotic insect, Polypedilum vanderplanki. We compare the genomes of P. vanderplanki and a congeneric desiccation-sensitive midge P. nubifer. We determine that the genome of the anhydrobiotic species specifically contains clusters of multi-copy genes with products that act as molecular shields. In addition, the genome possesses several groups of genes with high similarity to known protective proteins. However, these genes are located in distinct paralogous clusters in the genome apart from the classical orthologues of the corresponding genes shared by both chironomids and other insects. The transcripts of these clustered paralogues contribute to a large majority of the mRNA pool in the desiccating larvae and most likely define successful anhydrobiosis. Comparison of expression patterns of orthologues between two chironomid species provides evidence for the existence of desiccation-specific gene expression systems in P. vanderplanki.
UR - http://www.scopus.com/inward/record.url?scp=84919651803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919651803&partnerID=8YFLogxK
U2 - 10.1038/ncomms5784
DO - 10.1038/ncomms5784
M3 - Article
C2 - 25216354
AN - SCOPUS:84919651803
VL - 5
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 4784
ER -