Comparative effects of a recombinant and a mutein type of granulocyte colony stimulating factor on the growth of Meth-A fibrosarcoma with 5-fluorouracil chemotherapy

Yoshinori Nio, Takahiro Shiraishi, Michihiko Tsubono, Hideki Morimoto, Chen Chiu Tseng, Kazuya Kawabata, Yoshikazu Masai, Manabu Fukumoto, Takayoshi Tobe

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The present study was designed to evaluate the effects of a recombinant human G-CSF (rhG-CSF) and a mutein G-CSF(KW-2228) on leucopenia and tumor growth in mice treated with 5-fluorouracil (5-FU). In normal mice, the number of leucocytes (white blood cell, WBC) reached the peak 12 hours after a single injection of either type of G-CSF and decreased to the normal level after 24 hours. Daily administration induced a continuous increase in the WBC count, however, administrations at intervals did not. Meth-A fibrosarcoma was subcutaneously inoculated into the backs of syngeneic BALB/c mice. The mice were treated with 5-FU alone or with G-CSFs. Chemotherapy with 5-FU alone resulted in leucopenia and an insignificant inhibition of tumor growth. The conjunctive administration of G-CSFs with 5-FU resulted in a significantly augmented inhibition of tumor growth, and leukopenia was not seen. This augmenting effect was more prominent with KW-2228. These results suggest that in 5-FU chemotherapy G-CSFs may be beneficial in restoring the number of leucocytes from leucopenic state and in augmenting the tumor inhibitory effect. Furthermore, KW-2228 may be more beneficial than the natural type rhG-CSF.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalBiotherapy
Volume4
Issue number2
DOIs
Publication statusPublished - 1992 Jun 1

Keywords

  • 5-FU
  • G-CSF
  • KW-2228

ASJC Scopus subject areas

  • Pharmacology

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