TY - JOUR
T1 - Comparative analysis of the brain transcriptome in a hyper-aggressive fruit fly, Drosophila prolongata
AU - Kudo, Ayumi
AU - Shigenobu, Shuji
AU - Kadota, Koji
AU - Nozawa, Masafumi
AU - Shibata, Tomoko F.
AU - Ishikawa, Yukio
AU - Matsuo, Takashi
N1 - Funding Information:
This study was supported by Japan Society for the Promotion of Science grant # 23128511, #25128702, and #26660263 to TM. The authors have no conflict of interest.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Aggressive behavior is observed in many animals, but its intensity differs between species. In a model animal of genetics, Drosophila melanogaster, genetic basis of aggressive behavior has been studied intensively, including transcriptome analyses to identify genes whose expression level was associated with intra-species variation in aggressiveness. However, whether these genes are also involved in the evolution of aggressiveness among different species has not been examined. In this study, we performed de novo transcriptome analysis in the brain of Drosophila prolongata to identify genes associated with the evolution of aggressiveness. Males of D. prolongata were hyper-aggressive compared with closely related species. Comparison of the brain transcriptomes identified 21 differentially expressed genes in males of D. prolongata. They did not overlap with the list of aggression-related genes identified in D. melanogaster, suggesting that genes involved in the evolution of aggressiveness were independent of those associated with the intra-species variation in aggressiveness in Drosophila. Although females of D. prolongata were not aggressive as the males, expression levels of the 21 genes identified in this study were more similar between sexes than between species.
AB - Aggressive behavior is observed in many animals, but its intensity differs between species. In a model animal of genetics, Drosophila melanogaster, genetic basis of aggressive behavior has been studied intensively, including transcriptome analyses to identify genes whose expression level was associated with intra-species variation in aggressiveness. However, whether these genes are also involved in the evolution of aggressiveness among different species has not been examined. In this study, we performed de novo transcriptome analysis in the brain of Drosophila prolongata to identify genes associated with the evolution of aggressiveness. Males of D. prolongata were hyper-aggressive compared with closely related species. Comparison of the brain transcriptomes identified 21 differentially expressed genes in males of D. prolongata. They did not overlap with the list of aggression-related genes identified in D. melanogaster, suggesting that genes involved in the evolution of aggressiveness were independent of those associated with the intra-species variation in aggressiveness in Drosophila. Although females of D. prolongata were not aggressive as the males, expression levels of the 21 genes identified in this study were more similar between sexes than between species.
KW - Gene expression levels
KW - Inter-species comparison
KW - RNA-seq analysis
KW - de novo transcriptome analysis
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U2 - 10.1016/j.ibmb.2017.01.006
DO - 10.1016/j.ibmb.2017.01.006
M3 - Article
C2 - 28115271
AN - SCOPUS:85010679683
VL - 82
SP - 11
EP - 20
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
ER -