Comparative analysis of bovine serum albumin adsorption onto octacalcium phosphate crystals prepared using different methods

Kaori Tsuchiya, Ryo Hamai, Susumu Sakai, Osamu Suzuki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.

Original languageEnglish
Pages (from-to)883-891
Number of pages9
JournalDental materials journal
Volume39
Issue number5
DOIs
Publication statusPublished - 2020

Keywords

  • Adsorption
  • Bovine serum albumin
  • Co-precipitation
  • Gelatin
  • Octacalcium phosphate

ASJC Scopus subject areas

  • Ceramics and Composites
  • Dentistry(all)

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