TY - JOUR
T1 - Comparative analysis of ATP-binding cassette (ABC) transporter gene expression levels in peripheral blood leukocytes and in liver with hepatocellular carcinoma
AU - Moustafa, Mohsen A.
AU - Ogino, Daisuke
AU - Nishimura, Masuhiro
AU - Ueda, Nobuhiko
AU - Naito, Shinsaku
AU - Furukawa, Motonobu
AU - Uchida, Takafumi
AU - Ikai, Iwao
AU - Sawada, Hideki
AU - Fukumoto, Manabu
N1 - Funding Information:
Acknowledgement. This work was supported by the Grant for State Key Program for Basic Research of China (973) No. 2013CB329405, National Natural Science Foundation (No. 61104214, No. 61203222), Specialized Research Fund for the Doctoral Program of Higher Education (20120201120036), Shaanxi Province Science and Technology Research and Development Program (2013KJXX-46), and Fundamental Research Funds for the Central Universities.
PY - 2004/6
Y1 - 2004/6
N2 - ATP-binding cassette (ABC) transporters comprise a superfamily of similar proteins involved in transmembrane transport of various substances. ABC transporter family members in the liver participate in bile formation and lipid metabolism. In order to assess whether peripheral blood leukocytes (PBL) are available as a surrogate for determination of the expression of ABC transporter genes in the liver, we compared ABC transporter gene expression levels in PBL with those in liver tissues from patients with hepatocellular carcinoma (HCC). We measured ABCA1, A2, B1-B4, C1°C5, G1 and G2 gene expression levels in PBL, and cancerous and non-cancerous portions of liver from patients with hepatocellular carcinoma by means of real time reverse-transcription (RT)-PCR. We could not detect ABCC5 expression in any tissue of the liver. Close correlations between ABCA2 C1 and G1 in PBL and in non-tumor tissues of the liver were found. Compared with the non-tumor part, HCC tissue expressed lower levels of ABCA1, B4 and G2. We think monitoring of ABCA2, C1 and G1 gene expression levels in PBL will be useful for selection of anti-cancer agents and monitoring of drug resistance of HCC. Administration of chemotherapeutic agents which are substrates of ABCA1, B4 and G2 should be effective for the treatment of HCC.
AB - ATP-binding cassette (ABC) transporters comprise a superfamily of similar proteins involved in transmembrane transport of various substances. ABC transporter family members in the liver participate in bile formation and lipid metabolism. In order to assess whether peripheral blood leukocytes (PBL) are available as a surrogate for determination of the expression of ABC transporter genes in the liver, we compared ABC transporter gene expression levels in PBL with those in liver tissues from patients with hepatocellular carcinoma (HCC). We measured ABCA1, A2, B1-B4, C1°C5, G1 and G2 gene expression levels in PBL, and cancerous and non-cancerous portions of liver from patients with hepatocellular carcinoma by means of real time reverse-transcription (RT)-PCR. We could not detect ABCC5 expression in any tissue of the liver. Close correlations between ABCA2 C1 and G1 in PBL and in non-tumor tissues of the liver were found. Compared with the non-tumor part, HCC tissue expressed lower levels of ABCA1, B4 and G2. We think monitoring of ABCA2, C1 and G1 gene expression levels in PBL will be useful for selection of anti-cancer agents and monitoring of drug resistance of HCC. Administration of chemotherapeutic agents which are substrates of ABCA1, B4 and G2 should be effective for the treatment of HCC.
UR - http://www.scopus.com/inward/record.url?scp=3042633767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042633767&partnerID=8YFLogxK
U2 - 10.1111/j.1349-7006.2004.tb03244.x
DO - 10.1111/j.1349-7006.2004.tb03244.x
M3 - Article
C2 - 15182435
AN - SCOPUS:3042633767
VL - 95
SP - 530
EP - 536
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 6
ER -