Comparative analyses in Lotus: The cytogenetic map of Lotus uliginosus Schkuhr

J. Ferreira, S. Mendes, M. Dall'Agnol, N. Sandal, S. Sato, A. Pedrosa-Harand

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A comparative cytogenetic map was built for Lotusuliginosus (2n = 12), expanding previous analyses that revealed intra- and interspecific chromosomal rearrangements in the model legume L. japonicus, L. filicaulis, and L. burttii. This species is positioned in a sister clade of the previously-mapped species and is proposed as one of the progenitors of L. corniculatus, the main forage crop of the genus. The cytogenetic map allowed the location of 12 genomic regions to be compared between these species. A high macrosynteny was revealed, but it was interrupted by a translocation involving chromosomes 3 and 5, a new rearrangement for the genus. Also, a transposition on chromosome 2 was found in L. japonicus 'Miyakojima'. Furthermore, changes in the number, size, and position of rDNA sites were observed, as well as an intraspecific size heteromorphism of the 5S rDNA site on L. uliginosus chromosome 6. The karyotype differences observed are proportional to the phylogenetic distance among these species.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalCytogenetic and Genome Research
Volume137
Issue number1
DOIs
Publication statusPublished - 2012 Aug 1
Externally publishedYes

Keywords

  • Chromosomal rearrangement
  • Comparative mapping
  • FISH
  • Lotus japonicus
  • Macrosynteny
  • Translocation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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