Compact fluidic system for functional assessment of pancreatic islets

Takeshi Hori, Kei Yamane, Takayuki Anazawa, Osamu Kurosawa, Hiroo Iwata

Research output: Contribution to journalArticle

Abstract

Transplantation of pancreatic islets is becoming a promising therapy for people with type I diabetes. In this study, we present a compact fluidic system that enables assessment of islet functionality ex vivo for efficient islet transplantation. The fluidic system includes a micromesh sheet-embedded chip. Islets can be loaded easily on the micromesh sheet and observed clearly by microscopy. Islets on the mesh sheet mainly remained in place during perfusion and did not get damaged by hydraulic pressure because of high porosity of the micromesh sheet. The fluidic system was assembled with a sample fraction chip of polydimethylsiloxane. The chip includes a channel and columns, both having surfaces that were super-hydrophilized so that solutions could flow smoothly within the chip by gravity. Using mouse pancreatic islets, a dynamic glucose-stimulated insulin secretion test was performed to examine the performance of the fluidic system. The system successfully analyzed levels and patterns of insulin secretion upon exposure of the islets to low- and high-glucose solutions in turns, thus demonstrating its capacity to assess islet functions more easily and cost-effectively.

Original languageEnglish
Article number91
JournalBiomedical microdevices
Volume21
Issue number4
DOIs
Publication statusPublished - 2019 Dec 1
Externally publishedYes

Keywords

  • Ex vivo analysis
  • Fraction chip
  • Islet-on-a-chip
  • Micromesh sheet
  • Pancreatic islets

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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