Communication between lymphatic and venous systems in mice

Lenan Shao, Kazu Takeda, Shigeki Kato, Shiro Mori, Tetsuya Kodama

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The lymphatic system in mice consists of lymphatic vessels and 22 types of lymph nodes. Metastatic tumor cells in the lymphatic system spread to distant organs through the venous system. However, the communication routes between the lymphatic and venous systems have not been fully elucidated. Here, we identify the communication routes between the lymphatic and venous systems in the axillary and subiliac regions of MXH10/Mo- lpr/. lpr inbred mice, which develop systemic swelling of lymph nodes up to 10. mm in diameter, allowing investigation of the topography of the lymph nodes and lymphatic vessels. Using a gross anatomy dissection approach, the efferent lymphatic vessels of the proper axillary lymph node were shown to communicate with the subclavian vein. Furthermore, we found that the thoracoepigastric vein, which connects the subclavian vein and inferior vena cava, runs adjacent to the subiliac and proper axillary lymph nodes, and receives venous blood from these lymph nodes routed through small branches. The direction of blood flow in the thoracoepigastric vein occurred in two directions in the intermediate region between the proper axillary lymph node and subiliac lymph node; one to the subclavian vein, the other to the inferior vena cava. This paper reveals the anatomy of the communication between the lymphatic and venous systems in the axillary and subiliac regions of the mouse, and provides new insights relevant to the investigation of the mechanisms of lymph node metastasis and cancer immunology, and the development of diagnostic and treatment methods for lymph node metastasis, including drug delivery systems.

Original languageEnglish
Pages (from-to)100-105
Number of pages6
JournalJournal of Immunological Methods
Volume424
DOIs
Publication statusPublished - 2015 Sep 1

Keywords

  • Blood flow
  • Communication
  • Lymphatic system
  • Venous system

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Communication between lymphatic and venous systems in mice'. Together they form a unique fingerprint.

Cite this