TY - JOUR
T1 - Common gene expression signatures in t(8;21)- and inv(16)-acute myeloid leukaemia
AU - Ichikawa, Hitoshi
AU - Tanabe, Kenji
AU - Mizushima, Hiroshi
AU - Hayashi, Yasuhide
AU - Mizutani, Shuki
AU - Ishii, Eiichi
AU - Hongo, Teruaki
AU - Kikuchi, Akira
AU - Satake, Masanobu
PY - 2006/11
Y1 - 2006/11
N2 - Human acute myeloid leukaemia (AML) involving a core-binding factor (CBF) transcription factor is called CBF leukaemia. In these leukaemias, AML1 (RUNX1, PEBP2αB, CBFα2)-MTG8 (ETO) and CBFβ (PEBP2β)-MYH11 chimaeric proteins are generated by t(8;21) and inv(16) respectively. We analysed gene expression profiles of leukaemic cells by microarray, and selected genes whose expression appeared to be modulated in association with t(8;21) and inv(16). In a pair-wise comparison, 15% of t(8;21)-associated transcripts exhibited high or low expression in inv(16)-AML, and 26% of inv(16)-associated transcripts did so equivalently in t(8;21)-AML. These common elements in gene expression profiles between t(8;21)- and inv(16)-AML probably reflect the situation that AML1-MTG8 and CBFβ-MYH11 chimaeric proteins affect a common set of target genes in CBF leukaemic cells. On the other hand, 38% of t(8;21)-associated and 24% of inv(16)-associated transcripts were regulated in t(8;21)- and inv(16)-specific manners. These distinct features of t(8;21)- and inv(16)-associated genes correlate with the bimodular structures of the chimaeric proteins (CBF-related AML1 and CBFβ portions, and CBF-unrelated MTG8 and MYH11 portions).
AB - Human acute myeloid leukaemia (AML) involving a core-binding factor (CBF) transcription factor is called CBF leukaemia. In these leukaemias, AML1 (RUNX1, PEBP2αB, CBFα2)-MTG8 (ETO) and CBFβ (PEBP2β)-MYH11 chimaeric proteins are generated by t(8;21) and inv(16) respectively. We analysed gene expression profiles of leukaemic cells by microarray, and selected genes whose expression appeared to be modulated in association with t(8;21) and inv(16). In a pair-wise comparison, 15% of t(8;21)-associated transcripts exhibited high or low expression in inv(16)-AML, and 26% of inv(16)-associated transcripts did so equivalently in t(8;21)-AML. These common elements in gene expression profiles between t(8;21)- and inv(16)-AML probably reflect the situation that AML1-MTG8 and CBFβ-MYH11 chimaeric proteins affect a common set of target genes in CBF leukaemic cells. On the other hand, 38% of t(8;21)-associated and 24% of inv(16)-associated transcripts were regulated in t(8;21)- and inv(16)-specific manners. These distinct features of t(8;21)- and inv(16)-associated genes correlate with the bimodular structures of the chimaeric proteins (CBF-related AML1 and CBFβ portions, and CBF-unrelated MTG8 and MYH11 portions).
KW - Chimaera protein
KW - Gene expression
KW - Microarray
KW - Transcription factor
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U2 - 10.1111/j.1365-2141.2006.06310.x
DO - 10.1111/j.1365-2141.2006.06310.x
M3 - Article
C2 - 16989659
AN - SCOPUS:33749237123
VL - 135
SP - 336
EP - 347
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -