Common amino acid sequences deduced from coding exons of the porcine FGF4 gene in two breeds and production of the encoded protein in Escherichia coli

Masayuki Kobayashi, Saiko Sugawara, Toshihiko Ito, Hitoshi Suzuki, Toshikiyo Takahashi, Jun Ichi Konishi, Masaki Kobayashi, Sho Sato, Yuki Mori, Kano Kasuga, Ikuo Kojima, Tomokazu Fukuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the development of mammalian embryos. Here we identified common amino acid sequences predicted from coding exons of the FGF4 gene in five pigs of two breeds, and HispFGF4, a 6× histidinetagged porcine FGF4, was produced in Escherichia coli. HispFGF4 was purified efficiently from the supernatant of cell lysate by heparin column chromatography. In a porcine embryonic fibroblast cell line, HispFGF4 showed significant mitogenic activities at concentrations as low as 0.001 nM (p < 0:01). To the best of our knowledge, this is the first report describing the complete nucleotide sequence of coding exons for the porcine FGF4 protein in two breeds, together with the production of a recombinant, bioactive porcine FGF4 derivative.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Escherichia coli
  • Fibroblast growth factor 4
  • Porcine
  • Recombinant
  • Structural gene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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    Kobayashi, M., Sugawara, S., Ito, T., Suzuki, H., Takahashi, T., Konishi, J. I., Kobayashi, M., Sato, S., Mori, Y., Kasuga, K., Kojima, I., & Fukuda, T. (2013). Common amino acid sequences deduced from coding exons of the porcine FGF4 gene in two breeds and production of the encoded protein in Escherichia coli. Bioscience, Biotechnology and Biochemistry, 77(1), 173-177. https://doi.org/10.1271/bbb.120698