Combined use of T1-weighted MRI and MRA for stereotaxic lesioning of the nonhuman primate brain: Application to the rhinal cortex

Xavier Blaizot, Kenichi Meguro, Claude Le Mestric, Jean Marc Constans, Dominique Luet, Jean Claude Baron, Chantal Chavoix

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Stereotaxic brain lesioning is widely used to develop experimental models of human brain disease in the nonhuman primate. To avoid intraoperative vascular complications such as intracranial hemorrhage, we developed a methodology that is easy to implement. This method combines T1-weighted magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA). This technique is applied to produce bilateral neurotoxic lesions of the rhinal cortex, a structure located medially in the temporal lobe, in eight baboons (including five sham-operated animals with needle descents but no ibotenic acid injection). Two other baboons were lesioned before the MRA technology was available. The MRA sequence (two-dimensional time-of-flight, axial acquisition) was used to localize the blood vessels in the needle trajectories, i.e., the highly vascularized sylvian fissure and temporal gyri. The vessel coordinates were transposed onto the coronal MRI-T1 images, onto which the injection sites were determined and the planned needle tracks drawn. In the eight baboons that had MRA, 26.8% of these needle tracks had to be slightly displaced because of the presence of blood vessels. The stereotaxic coordinates of the final target sites were then calculated with respect to six skull landmarks that also served as a reference during surgery. No intracranial hemorrhage occurred in any of the eight baboons in which MRA was performed, in contrast to one of the two baboons not studied with MRA. The histological analysis showed a good extent of the rhinal lesions in all lesioned animals, with minimal damage to areas other than those that were targeted. Thus, combined use of MRI-T1 and MRA proved to be reliable in reducing vascular complications, affording new advances for stereotaxic surgery in nonhuman primates.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalExperimental Brain Research
Issue number1
Publication statusPublished - 1999
Externally publishedYes


  • Magnetic resonance angiography
  • Magnetic resonance imaging
  • Nonhuman primate
  • Rhinal cortex
  • Stereotaxic surgery

ASJC Scopus subject areas

  • Neuroscience(all)


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