Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster

Hiroko Iwasaki, Haorile Chagan-Yasutan, Prisca Susan A. Leano, Nobuo Koizumi, Chie Nakajima, Delsi Taurustiati, Firmanto Hanan, Talitha Lea Lacuesta, Yugo Ashino, Yasuhiko Suzuki, Nina G. Gloriani, Elizabeth Freda O. Telan, Toshio Hattori

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated isothermal amplification (LAMP) and real-time PCR in plasma and 2 types of urine pellets. Of 113 suspected cases, 68.1%, 76.1%, and 60.2% were positive by MAT, ELISA, and ICT, respectively. Real-time PCR using DNA purified from urine pellets collected by low-speed centrifugation yielded positive signals for patients in late acute as well as early phase who were positive by LAMP using plasma DNA or urine pellets. Among antibody-negative patients, 9.5% were positive by DNA detection. These findings indicate that the leptospirosis detection rate is increased by combining antibody and DNA detection, providing a new tool for timely diagnosis of infection.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume84
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Keywords

  • Antibody detection
  • DNA detection
  • Disaster
  • Leptospirosis
  • Urine

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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