Combined analyses of bacterial, fungal and nematode communities in andosolic agricultural soils in Japan

Zhihua Bao, Yoko Ikunaga, Yuko Matsushita, Sho Morimoto, Yuko Takada-Hoshino, Hiroaki Okada, Hirosuke Oba, Shuhei Takemoto, Shigeru Niwa, Kentaro Ohigashi, Chika Suzuki, Kazunari Nagaoka, Makoto Takenaka, Yasufumi Urashima, Hiroyuki Sekiguchi, Atsuhiko Kushida, Koki Toyota, Masanori Saito, Seiya Tsushima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We simultaneously examined the bacteria, fungi and nematode communities in Andosols from four agro-geographical sites in Japan using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and statistical analyses to test the effects of environmental factors including soil properties on these communities depending on geographical sites. Statistical analyses such as Principal component analysis (PCA) and Redundancy analysis (RDA) revealed that the compositions of the three soil biota communities were strongly affected by geographical sites, which were in turn strongly associated with soil characteristics such as total C (TC), total N (TN), C/N ratio and annual mean soil temperature (ST). In particular, the TC, TN and C/N ratio had stronger effects on bacterial and fungal communities than on the nematode community. Additionally, two-way cluster analysis using the combined DGGE profile also indicated that all soil samples were classified into four clusters corresponding to the four sites, showing high site specificity of soil samples, and all DNA bands were classified into four clusters, showing the coexistence of specific DGGE bands of bacteria, fungi and nematodes in Andosol fields. The results of this study suggest that geography relative to soil properties has a simultaneous impact on soil microbial and nematode community compositions. This is the first combined profile analysis of bacteria, fungi and nematodes at different sites with agricultural Andosols.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalMicrobes and environments
Volume27
Issue number1
DOIs
Publication statusPublished - 2011

Keywords

  • Andosols
  • Bacteria-fungi-nematode community
  • PCR-DGGE
  • Two-way cluster analysis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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    Bao, Z., Ikunaga, Y., Matsushita, Y., Morimoto, S., Takada-Hoshino, Y., Okada, H., Oba, H., Takemoto, S., Niwa, S., Ohigashi, K., Suzuki, C., Nagaoka, K., Takenaka, M., Urashima, Y., Sekiguchi, H., Kushida, A., Toyota, K., Saito, M., & Tsushima, S. (2011). Combined analyses of bacterial, fungal and nematode communities in andosolic agricultural soils in Japan. Microbes and environments, 27(1), 72-79. https://doi.org/10.1264/jsme2.me11281