Combinations of three antimicrobial agents. (i). Experimental three-dimensional checkerboard dilution method for quantitative analysis of the in vitro combined effect of three antimicrobial agents

Akira Watanabe, Kotaro Oizumi, Kiyoshi Konno

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    We have established an experimental three-dimensional checkerboard dilution method for quantitative analysis of the in vitro combined effect of three antimicrobial agents. We designed this method through a modification of the checkerboard dilution method used for two antimicrobial agents. In this experiment, we used a micro-broth dilution method using the Dynatech MIC-2000 system. A two-fold serial dilution of a third agent was added to the checkerboard containing the first and second agents. Then, 0.0015 ml of a bacterial suspension was inoculated and incubated for 24 hours at 37°C. The results obtained were plotted on a three-dimensional cubic graph so as to describe a spherical surface, and the volume under the surface (VUS) was calculated. By comparing the VUS, with the relative proportion of the three agents fixed at a certain ratio, it was possible to compare the efficacy of triple antimicrobial combinations. For example, by application of the three-dimensional checkerboard method, we could evaluate the effect of the combination of a combined agent with a single agent, or the combination of equal amounts of three agents.

    Original languageEnglish
    Pages (from-to)613-616
    Number of pages4
    JournalChemotherapy
    Volume36
    Issue number9
    DOIs
    Publication statusPublished - 1988 Jan 1

    ASJC Scopus subject areas

    • Pharmacology (medical)
    • Infectious Diseases
    • Pharmacology
    • Drug Discovery
    • Oncology

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