TY - JOUR
T1 - Combination of Conventional and Endotoxin-Specific Limulus Tests for Measurement of Polysaccharides in Sera of Rabbits with Experimental Systemic Candidiasis
AU - Miyazaki, Takashige
AU - Kohno, Shigeru
AU - Mitsutake, Kotaro
AU - Yamada, Hiroshi
AU - Yasuoka, Akira
AU - Maesaki, Shigefumi
AU - Kaku, Mitsuo
AU - Koga, Hironobu
AU - Hara, Kohei
PY - 1992
Y1 - 1992
N2 - Factor G, the coagulation enzyme from limulus amebocytes, is activated by (1→3)-β-D-glucan but not by endotoxin. The endotoxin-specific limulus test, which is devoid of fator G, reacts only with endotoxin. However, the conventional limulus test which includes factors G and C, reacts with not only endotoxin but also with (1→3)-β-D-glucan. In this study, the culture supernatant of Candida albicans in RPMI medium activated the conventional limulus test, but not the endotoxin-specific limulus test. All five rabbits inoculated intravenously with Candida albicans (1.0×107 CFU/rabbit) showed increased levels of reactivity with the conventional limulus test, whereas no elevation in the levels of the endotoxin-specific limulus test was observed in the sera of any infected rabbits. Only one serum sample from rabbit No. 5 on day 8 showed a positive Cand-tectest. The difference in the titers of the two limulus tests was suggestive of a diagnosis of Candida infection.-limulus test; serological diagnosis; β-glucan; polysaccharide; candidiasis.
AB - Factor G, the coagulation enzyme from limulus amebocytes, is activated by (1→3)-β-D-glucan but not by endotoxin. The endotoxin-specific limulus test, which is devoid of fator G, reacts only with endotoxin. However, the conventional limulus test which includes factors G and C, reacts with not only endotoxin but also with (1→3)-β-D-glucan. In this study, the culture supernatant of Candida albicans in RPMI medium activated the conventional limulus test, but not the endotoxin-specific limulus test. All five rabbits inoculated intravenously with Candida albicans (1.0×107 CFU/rabbit) showed increased levels of reactivity with the conventional limulus test, whereas no elevation in the levels of the endotoxin-specific limulus test was observed in the sera of any infected rabbits. Only one serum sample from rabbit No. 5 on day 8 showed a positive Cand-tectest. The difference in the titers of the two limulus tests was suggestive of a diagnosis of Candida infection.-limulus test; serological diagnosis; β-glucan; polysaccharide; candidiasis.
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U2 - 10.1620/tjem.168.1
DO - 10.1620/tjem.168.1
M3 - Article
C2 - 1488754
AN - SCOPUS:0026921259
VL - 168
SP - 1
EP - 9
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 1
ER -